Simonet et al.'s*

FTW Medium


Component
Per liter
Glucose
10 g
Casein Hydrolyzate
4 g
Yeast Extract
0.050
Tween 80
2 ml

K2HPO4

0.50 g

MgSO4.7H2O
0.200 g 
CaCl2.2H2O  
0.003 g
Citric acid
0.010 g
Fe.citrate 
0.010 g
Microelements
1.0 ml
Vitamin Stock
1.0 ml

(adjust pH to 6.7)


*Simonet, P., P. Normand, A. Moiroud and M. Lalonde. 1985. Restriction enzyme digestion of Frankia plasmids. Plant and Soil 87:49-60).

Vitamin Stock:

Component
g/l
Nicotinic Acid
0.5
Pyridoxin
0.5
Thiamin
0.1

 

Microelements:

Component
(g/l)
H3BO4
1.5 g
MnCl2.4H2O
0.7 g
ZnSO4.7H2O
0.6 g
CuSO4.5H2O
0.1 g
(NH4)6Mo7O24
0.020 g
CoCl2.6H2O
0.001