Baker and O'Keefe's

Frankia Defined Propionate Minimal Medium (DPM)

For routine growth and subculture of Frankia


 

 

Component
Per liter
NaPropionate
1.2 g
KH2PO4
1.0 g
MgSO4.7H2O
0.1 g 
CaCl2.2H2O  
0.01
FeSO4.7H2O/EDTA stock  
1.0 ml
Hoagland's microelements
1.0 ml

(adjust pH to 6.5)


This medium was used initially as N-deficient medium for isolating Frankia from nodules and soil. Ammonium chloride can be added (0.5 g/l) to make it complete (DPM+N) for growth of Frankia strains in culture.

1000X Stock Iron: Dissolve 0.75 g Na2EDTA.2H2O, 0.56 g FeSO4.7H2O ( make up to 100 ml).

 

Hoagland's Microelements:

Component
(g/l)
H3BO4
2.8 g
MnCl2.4H2O
1.8 g
ZnSO4.7H2O
0.2 g
CuSO4.5H2O
0.1 g
NaMoO4
0.025 g

Notes:  - Proteose Peptone #3 can be added at a level of 0.5 g/l for some growth improvement.  

- Although more expensive, MOPS can be added to better control pH. Make up a 10X NaMOPS solution to 200 mM, pH6.5 by adding 46 g to 1 liter dH2O and then add at a rate of 100 ml per liter, keeping all other weighed components the same.