Murry, Fontaine & Torrey's

BAP Medium

For routine growth and subculture of Frankia


 

 

Component
Per liter
NaPropionate
0.5 g
KH2PO4
0.95 g

K2HPO4

0.60 g

MgSO4.7H2O
0.025 g 
CaCl2.2H2O  
0.003 g
Biotin
450 mg
FeSO4.7H2O/EDTA stock  
1.0 ml
Hoagland's microelements
1.0 ml

(adjust pH to 6.7)


This medium was used initially for growth experiments of HFPArI3 (Murry et al., 1984. Plant and Soil 78:61). It can be used for routine maintenance of strains in culture. The C-source was initially filter sterilized but is not necessary in the case of propionate.

1000X Stock Iron: Dissolve 0.75 g Na2EDTA.2H2O, 0.56 g FeSO4.7H2O ( make up to 100 ml).

 

Hoagland's Microelements:

Component
(g/l)
H3BO4
2.8 g
MnCl2.4H2O
1.8 g
ZnSO4.7H2O
0.2 g
CuSO4.5H2O
0.1 g
NaMoO4
0.025 g