Murry, Fontaine & Torrey's
BAP Medium
For routine growth and subculture of Frankia
Component |
Per liter |
NaPropionate |
0.5 g |
KH2PO4 |
0.95 g |
K2HPO4 |
0.60 g |
MgSO4.7H2O |
0.025 g |
CaCl2.2H2O |
0.003 g |
Biotin |
450 mg |
FeSO4.7H2O/EDTA stock |
1.0 ml |
Hoagland's microelements |
1.0 ml |
This medium was used initially for growth experiments of HFPArI3 (Murry et al., 1984. Plant and Soil 78:61). It can be used for routine maintenance of strains in culture. The C-source was initially filter sterilized but is not necessary in the case of propionate.
1000X
Stock Iron: Dissolve 0.75 g Na2EDTA.2H2O, 0.56 g FeSO4.7H2O
Hoagland's Microelements:
Component |
(g/l) |
H3BO4 |
2.8 g |
MnCl2.4H2O |
1.8 g |
ZnSO4.7H2O |
0.2 g |
CuSO4.5H2O |
0.1 g |
NaMoO4 |
0.025 g |