Diagnostic Genetic Sciences (DGS)
Diagnostic Genetic Sciences Program Director: Martha B. Keagle
Program Office: Room 222, Koons Hall
For major requirements, see the College of Agriculture and Natural Resources, Department of Allied Health Sciences section of this Catalog.
222. Medical Cytogenetics
Both semesters. Four credits. Two 2-hour lectures. Prerequisite: MCB 203 and 200 or 213; all of which may be concurrent. Open to students in the Diagnostic Genetic Sciences Program; others who have met the prerequisites.
Birth defects, prenatal assessment, cell culture and harvest, staining and banding techniques, mechanisms of numerical and structural chromosome abnormality, numerical syndromes, duplication and deletion syndromes, the sex chromosomes, sex chromosome abnormalities, human chromosome nomenclature, mosaicism, genetic imprinting, cancer cytogenetics, molecular cytogenetic testing.
223. Laboratory in Cytogenetics
Both semesters. Two credits. One 3-hour laboratory period and one 1-hour discussion. Four additional laboratory sessions are required during the first half of the semester. Prerequisite: DGS 222 which may be taken concurrently. Open only to students enrolled in the Diagnostic Genetic Sciences Program; others with consent of instructor.
Human chromosome morphology and identification, aseptic technique, lymphocyte culture and harvest, chromosome banding, karotyping and microscopic analysis of normal and abnormal cases.
224. Cancer Cytogenetics
Offered summer. Two credits. Prerequisite: DGS 223. Open only to students enrolled in the Diagnostic Genetic Sciences Program; others with consent of instructor.
Chromosome instability syndromes, genetic basis of cancer, cytogenetics of solid tumors and hematologic malignancies, and nomenclature of acquired changes.
225. Microscopy and Chromosome Imaging
Either semester. One credit. Prerequisite: DGS 223 which may be taken concurrently. Open only to students enrolled in the Diagnostic Genetics Sciences Program; others with consent of instructor. Brown
Theory and techniques of brightfield and fluorescence microscopy,
and computerized chromosome imaging.
226. Current Genetic Research
Either semester. Variable credits. Prerequisite: DGS 222 or MCB 200 or MCB 213; instructor consent. May be repeated one time for credit.
Retrieval, review and discussion of current primary genetics literature in addition to attending and reviewing University research seminars/guest speakers.
234. Diagnostic Molecular Technologies
Both semesters. Three credits. Prerequisite: MCB 200 or 213; and MLS 208 or MCB 211 which may be taken concurrently. Open only to students enrolled in the Diagnostic Genetic Sciences Program.
DNA and RNA diagnostic technologies used in clinical settings; clinical applications in prenatal diagnosis; cancer management, transplantation, paternity testing, forensic medicine and microbiology.
234W. Diagnostic Molecular Technologies
Prerequisite: MCB 200 or 213; and MLS 208 or MCB 211 which may be taken concurrently; ENGL 105 or 110 or 111 or 250. Open only to students enrolled in the Diagnostic Genetic Sciences Program.
235. Laboratory in Molecular Diagnostics
Both semesters. Two credits. Prerequisite: DGS 234 or MLS 217 which may be taken concurrently. Open only to students enrolled in the Diagnostic Genetic Sciences Program.
DNA isolation, blotting techniques, fluorescent in situ hybridization, polymerase chain reaction and Genprobe assay.
246. Contemporary Issues in Human Genetics
Both semesters. Three credits. Prerequisite: DGS 223. Open only to students enrolled in the Diagnostic Genetic Sciences Program; others with consent of the instructor.
Advanced karotyping and microscopic diagnosis; report writing; historical perspective; recent advances and future trends in human genetics; ethical issues of genetic research, technological advances, genetic diagnosis and the practice of medical genetics; exploration of the lay person's understanding of human genetics and genetic diagnosis.
250. Specimen Processing
Both semesters. Two credits. Prerequisite: In order to enroll in this course, a student must have earned a "C" or better in DGS 234 and 235. Open to DGS molecular students only.
Theory and methods for processing and evaluating specimens for nucleic acid isolation.
251. Nucleic Acid Isolation
Both semesters. Four credits. Prerequisite: In order to enroll in this course, a student must have earned a "C" or better in DGS 234 and 235. Open to DGS molecular students only.
Isolation, manipulation and evaluation of nucleic acids.
252. Cloning Techniques
Both semesters. Two credits. Prerequisite: In order to enroll in this course, a student must have earned a "C" or better in DGS 234 and 235. Open to DGS molecular students only.
Theory and techniques of cloning.
253. Blotting Applications
Both semesters. Two credits. Prerequisite: In order to enroll in this course, a student must have earned a "C" or better in DGS 234 and 235. Open to DGS molecular students only.
Theory and techniques of nucleic acid and/or protein blotting (e.g. Southern blot, reverse clot blot).
261. Amplification Methods
Both semesters. Six credits. Prerequisite: In order to enroll in this course, a student must have earned a "C" or better in DGS 234 and 235. Open only to DGS molecular students only.
Practicum experience in DNA and/or RNA amplification stressing polymerase chain reaction.
273. Research in Molecular Genetics
Both semesters. One credit. Prerequisites: In order to enroll in this course, a student must have earned a "C" or better in AH 241W, DGS 234 and DGS 235. Open only to DGS molecular students only.
Design and implementation of a research project in molecular genetics.
275. In Situ Hybridization Methods
Both semesters. Two credits. Prerequisites: In order to enroll in this course, a student must have earned a "C" or better in DGS 234 and 235. Open to DGS molecular students only.
Practicum in fluorescence in situ hybridization or other in situ hybridization techniques.
276. Topics in Molecular Genetics
Both semesters. Two credits. Prerequisite: In order to enroll in this course, a student must have earned a "C" or better in DGS 234 and DGS 235. Open to DGS molecular students only.
Exploration of an individual area of interest in molecular genetics.
278. DNA Sequencing
Both semesters. Two credits. Prerequisite:In order to enroll in this course, a student must have earned a "C" or better in DGS 234 and DGS 235.
Open to DGS molecular students only.
Practicum experience in DNA sequencing.
279. Microbiological Applications of Molecular Diagnostics
Both semesters. Two credits. Prerequisite: In order to enroll in this course, a student must have earned a "C" or better in DGS 234 and DGS 235. Open only to students enrolled in the Molecular Diagnostic Sciences Program; others with consent of the instructor.
Practicum experience in the application of molecular technologies to microbiology.
280. Bone Marrow Cytogenetics
Both semesters. Three credits. Prerequisite: In order to enroll in the course, the student must have earned a "C" or better in DGS 222, DGS 223 and DGS 224. Open only to Diagnostic Genetic Sciences majors.
Culture, harvest, banding and analysis of leukemic bone marrow samples; chromosomal abnormalities associated with hematologic malignancies.
281. Peripheral Blood Cytogenetics
Both semesters. Four credits. Prerequisite: In order to enroll in this course, the student must have earned a "C" or better in DGS 222 and DGS 223. Open only to Diagnostic Genetic Sciences majors.
Culture, harvest, banding and analysis of peripheral blood samples.
284. Variable Topics in Cytogenetics
Both semesters. One credit. Prerequisite: In order to enroll in this course, the student must have earned a "C" or better in DGS 222 and DGS 223. Open only to Diagnostic Genetic Sciences majors.
In-depth examination of a topic of the students' choosing in the field of human genetics.
285. Research in Cytogenetics
Both semesters. One credit. Prerequisite: In order to enroll in this course, the student must have earned a "C" or better in AH 241W, DGS 222 and 223. Open only to Diagnostic Genetic Sciences majors.
Design and implementation of a research project in clinical cytogenetics.
286. Prenatal Cytogenetics
Both semesters. Four credits. Prerequisite: In order to enroll in this course, the student must have earned a "C" or better in DGS 222 and DGS 223. Open only to Diagnostic Genetic Sciences majors.
Culture, harvest, banding and analysis of amniotic fluids, products of conception, and other fetal samples.
287. Practicum in Microscopy and Imaging
Either semester. One credit. Prerequisite: To enroll in this course, the student must have earned a "C" or better in DGS 222 and 225. Open only to Diagnostic Genetic Sciences majors. Students taking this course will be assigned a grade of S (satisfactory) or U (unsatisfactory).
Brightfield microscopy, fluorescence microscopy and computer imaging. Student will become proficient in karyotyping skills through regular use of a computer imaging system.
288. Practicum in Staining and FISH
Both semesters. One credit. Prerequisite: To enroll in this course, the student must have earned a "C" or better in DGS 222 and 223. Open only to Diagnostic Genetic Sciences majors. Students taking this course will be assigned a grade of S (satisfactory) or U (unsatisfactory). Brown
Traditional routine and special cytogenetic staining techniques and fluorescence in situ hybridization (FISH).
289. DNA Technology in Forensic Science
Either semester. Credits and hours by arrangement. Prerequisite: To enroll in this course, the student must have earned a "C" or better in DGS 260, 261, and 273. Open only to Diagnostic Genetic Sciences molecular diagnostic sciences certificate students. Brown
Practicum experience with forensic applications of DNA testing.
290. Honors Research
Either semester. Three credits. Open only to Diagnostic Genetic Sciences Honors students.
Design and implementation of an honors research project.
298. Special Topics
Either semester. Credits and hours by arrangement. Open only with consent of instructor. May be repeated for credit.
Application of the scientific method of inquiry to planning, implementation, evaluating and reporting a study of a problem in cytogenetics.
299. Independent Study for Undergraduates
Either semester. Credits and hours by arrangement. Open only with consent of instructor. May be repeated for credit.
This course is designed primarily for students who wish to extend their knowledge in some specialized area in the field of diagnostic genetic sciences.
